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The Role of Neutrophils in Alpha-1 Antitrypsin Deficiency

Samples were sectioned using an ultramicrotome (Leica EM UC6) to 6090 nm, transferred to copper grids and left to dry before imaging

Moreover, viral vector gene transfer has been tested pre-clinically and is more promising because it provides a more efficient transcription transfer than non-viral methods

Ancillary Information Supporting Information Comparison of pulldown results with previously published datasets

NMR validation of 1907 interactions and circular dichroism To validate the binding mode of 1907 and map its interactions with the FAT domain, we performed heteronuclear single quantum coherence-nuclear magnetic resonance (HSQC-NMR) chemical shift perturbation (CSP) studies with 15 N labeled recombinant FAT protein and titrating amounts of 1907 from 100 nM to 200 M (Fig