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The You should not use a medication after its expiration date as it can be less effective and have an increased risk of safety issues

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Functional genomic analysis of frataxin deficiency reveals tissue-specific alterations and identifies the PPARgamma pathway as a therapeutic target in Friedreichs ataxia

This keeps the math identical to every other vial size using the base-10 approach, where each 10 units equals 1mg

The correct calculation requires converting 2.5mg/mL to 2500mcg/mL first, yielding 250mcg 2500mcg/mL = 0.1mL