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Take the appropriate solution to prepare the standards with the concentration of 200 g/mL, 100 g/mL, 50 g/mL, 25 g/mL and 12.5 g/mL (Dilute Reagent I ten times before diluting the standard solution)

However, changes in body fat and metabolism can influence estradiol levels over time

and (v) differences in blinding and reporting standards

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Future studies should explore rational combination strategies that incorporate CSC-targeting agents with immune checkpoint inhibitors, metabolic inhibitors, or chemotherapies to ensure that CSCs are eradicated alongside bulk tumor cells