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After incubation, the coverslip was transferred into the original medium for 15 min and for labeling, neurons were three times washed with 1x PBS and fixed with 4 % PFA/ 4 % sucrose in PBS for 15 min at room temperature (RT)

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Specifically, lower concentrations of lycopene and non-vitamin A carotenoids were observed in atopic infants, suggesting that lycopene may provide protection against atopy (119)

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