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This step is often the most challenging, emotionally taxing, and financially burdensome part of the journey, but functional medicine practitioners universally agree it is non-negotiable

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The solution was removed, replaced with 30 L of media containing Balifomycin A1 (1:1000 dilution) and SPiDER-Gal (1:500 dilution), and incubated for 45 min at 37 C

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