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Refinement was performed using Phenix 54 and Refmac5 55,56 , coupled with rounds of manual model building, real-space refinement and structure validation performed using COOT 57

The topGO R library [22] was used to determine local similarities and dependencies between GO terms to perform Elim pruning correction

And so they need a little bit more than someone else on average, they're the effects are the same