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C After mild regeneration wash Conditions Stationary phase: Kromasil 100-10-C4 Mobile phase solvent: acetonitrile Mobile phase salt: ammonium acetate Pool analysis, 95% purity Conditions Phase: Kromasil 100-3.5-C4 Eluent: acetonitrile / water Eluent salt: trifluoroacetic acid After the regeneration wash, peak shape and thereby efficiency and purity has been practically restored

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In T1D, autoreactive T cells develop in the pancreatic lymph nodes, where dendritic cells and, eventually, B cells, serve as antigen-presenting cells (APCs) to CD4+ T cells via MHC class II proteins ( Although autoreactive T cells are a hallmark of T1D, preproinsulin-specific CD8+ T cells are present at comparable levels in the exocrine pancreas of healthy, AAb+, and T1D patients ( high autoreactive memory T cells have also been reported in the pancreatic lymph nodes of NOD mice

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