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Plant Cell Environ 48:37783792

The postnuclear lysates were loaded on streptavidin beads that had been preloaded with Trx1 trapping mutant (Trx1 C35S SBP 6x His)

The reaction (10 l) began by incubating RAD51 (2.7 M) with 80 nM ssDNA immobilized on magnetic beads in Buffer N (35 mM Tris, pH 7.5, containing 1 mM DTT, 0.01% Igepal, 2 mM AMP-PNP, 1 mM MgCl2, 50 mM KCl, 100 ng/l BSA) for 5 minutes at 37 C

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