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coli s turgor pressure has been estimated to be ~1 atm 19 , consistent with inferred values based on our AFM measurements (see Methods for details), these observations suggest that decreases in effective stiffness, arising from the loss of turgor pressure during cytoplasmic condensation, may be offset by the intracellular compactification of macromolecules suggested by phase-contrast and fluorescence microscopy measurements (Supplementary Fig

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