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Any misstep in the mixing process can degrade the peptides, alter their concentrations, and ultimately render an entire experiment's data useless
(By the way, this is precisely what makes HILIC an orthogonal technique to RPLC!) By contrast, retention in many HILIC columns depends on the semi-immobilized water layer formed at the interface of the polar stationary phase and the organic-rich mobile phase
Macrovascular disease is also a major concern in type 2 diabetes mellitus (T2DM) treatment
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