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Learning latency was significantly pronounced in both male and female mice treated with intranasal saline, with much slower escape times and a lower magnitude of improvement, validating the naturally occurring cognitive decline of these mice

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Then, the cells were washed with PBS and lysed with PTTS buffer (0.5% Triton-100, 0.1% Tween-20, and 0.05% SDS) containing a protease inhibitor (Cat