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Then they further optimized the design and synthesized two kinds of lactam-stapled peptides to study their properties on helicity, bioactivity, and cell uptake, in which the lactam-stapled peptide galactose-6-phosphate isomerase subunit (LacB)-4E-BP1 exhibits extremely high cellular uptake efficiency (84% 20%) that is sufficient to bind to the cell membrane, trigger endocytosis and subsequently lead to endosome escape [162]

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