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For each qPCR reaction, 25 l of master mix was prepared by adding 12.5 l of the 2 Universal SYBR (ThermoFisher) 5.5 l of RNAse-free water, 1 l ACX primer (0.05 g /ul), 1 l TS primer (0.1 g /l) (Table 1), and 4 l of the protein sample
Deciphering the molecular determinants of VCs context-dependent anticancer effects may accelerate its rational clinical deployment
This retrofusion process, in which intraluminal vesicles (ILVs) fuse with the endosomal membrane, is beginning to be elucidated 62,63
Mild allergic reactions, limited to localized redness and itching, can often be managed at home with over-the-counter hydrocortisone cream and oral antihistamines