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For significant associations, we tested the potential explanatory effect of lifestyle and health-related variables by further including alcohol consumption, smoking status, physical activity, BMI and the number of self-reported current somatic diseases in the analytical models

The protein pellet was analyzed using Pierce BCA Protein Assay kit (Thermofisher) to determine protein concentrations, and the supernatant was transferred into a new tube for the identification of metabolite levels by mass spectrometry analysis at the Metabolomics Expertise Center (MEC - KU Leuven/VIB)

or any other sudden or unusual changes in behavior

Common subcutaneous injection sites: Abdomen Upper arm Thigh Rotate sites weekly to reduce the risk of irritation

A patch with plant extracts cant replicate that