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As LPS enhanced the phosphorylated Ser42 and Tyr198 in GSTP, we transfected GSTP mutants including GSTP-S42A, GSTP-S42D, GSTP-Y198F, GSTP-Y198D, and GSTP-S184D into RAW264.7 cells and then treated cells with LPS for 18 h

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( Tnf, Il6, Il1b ) and downregulates pro-fibrotic genes ( Acta2, Col1a2, Tgfb1, Pdgfrb, Pdgf-b, Pdgf-c ), reducing -smooth muscle actin (-SMA) and platelet-derived growth factor receptor beta (PDGFR) protein levels (73)

doi:10.1159/000123952 Banks WA, Kastin AJ, Coy DH, Angulo E

The U.K.s National Institute for Biological Standards and Control recommends a storage temperature of 4C, or 39F,29 so a refrigerator would be the ideal storage environment