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For testing the difference in mean and median lifespan between groups, we used linear mixed model with linear quantile mixed-effects model, which included cage assignment as a random effect and batch as a covariate, respectively, using package nlme and lgmm in statistical software R

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PT-141 is used on an as needed basis

Use a Fresh, Sterile Syringe for Every Step: Never, ever reuse a syringe or needle

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