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Newsome, Rohit Loomba Journal of Clinical Investigation .2025;[Epub] CrossRef High Prevalence of Fatty Liver in Bangladeshi Adolescents and Young Adults with Type 2 Diabetes: Key Predictors and Screening Recommendations Kishore Shil, Mashfiqul Hasan, Nusrat Sultana, Sayad Salam, Syeda Mostafa, Muhammad Hasanat Diabetes, Metabolic Syndrome and Obesity .2025

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Liver nuclei were isolated using a lysis buffer containing 10mM Tris-Hc pH 7.5, 10mM NaCl2, 3mM MgCl2, 0.05% Triton X, 45uM Actinomycin D, 0.2U/ul SuperaseIn (AM2694, ThermoFisher), and 0.1U/ul RNAse Inhibitor (Y9240L, Qiagen)

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